Download primary structure of protein10/31/2023 The methods to determine the C-terminal residue of the primary structure are as follows: The identification of phenylthiohydantoin is done by using chromatography. The isomerization of thiazolinone derivatives into phenylthiohydantoin is done by treating it with aqueous acid. This derivative is then reacted with a strong acid that forms a thiazolinone derivative. This reaction leads to the formation of a phenyl thiocarbamate. This method involves the reaction of phenyl isothiocyanate with a polypeptide chain. This derivative is then identified by fluorometric methods. This treatment results in a fluorescent derivative which is isolated by hydrolysis. In this method, the polypeptide chain is treated with a reagent named Dansyl chloride. The identification of this derivative is made by using chromatography or by comparing it with standards. A DNP derivative results after the acid hydrolysis of the polypeptide. Sanger reagent that is 1-fluoro-2,4-dinitrobenzene is reacted with a polypeptide chain, and this labels the N-terminal amino acid residue. This method involves stepwise removal and identification of amino acids from the N-terminal of the polypeptide chain. Different methods to identify the N-terminal end are as follows: This artificial synthesis of proteins requires the pre-knowledge of the amino acid sequence in protein structure.Īnalysis of end groups of the polypeptide is done to determine the structure of a primary protein. Sometimes, due to lack of natural proteins, these are made artificially by using applications of biotechnology. The biochemical function of a protein is dictated by the sequence of amino acids arranged in a polypeptide chain. Proteins work as enzymes, hormones, and take part in other essential processes of life. Proteins play an important role in our day to day life.
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